Journal: International Journal of Molecular Sciences

Article Title: Novel Benzoxazoles Containing 4-Amino-Butanamide Moiety Inhibited LPS-Induced Inflammation by Modulating IL-6 or IL-1β mRNA Expression

doi: 10.3390/ijms23105331

Figure Lengend Snippet: Compounds 5f and 4d attenuate inflammation in vitro. Effects of compounds (10 µM) on the protein levels of STAT3, IκB, and NF-κB in AML-12 cells. ( a ) Expression levels of inflammation related-proteins in vitro. ( b – d ) Protein expression levels were normal-ized against the indicated protein. * p < 0.05 compared with the group treated with the PBS (vehicle). + p < 0.05 compared with the group treated with the LPS. Data are pre-sented as the mean ± SD.

Article Snippet: The human keratinocytes HaCaT or the alpha mouse liver 12 (AML-12) cells were obtained from Cell Lines Service GmbH (Eppelheim, Germany).

Techniques: In Vitro, Expressing

Journal: International Journal of Stem Cells

Article Title: Peptides Targeting Fms-Related Tyrosine Kinase-4 Activate the Function of Natural Killer Cells in Acute Myeloid Leukemia

doi: 10.15283/ijsc21083

Figure Lengend Snippet: The effects of peptide targeted FLT-4 in leukemic mouse model. (A) Expressions of NK cells and IFN-γ of NK cells in BM after single, double, and triple treatments (n=12). (B) Expressions of NK cells and IFN-γ of NK cells in spleen after single, double, and triple treatments (n=12). (C) Expressions of NK cells and IFN-γ of NK cells in liver after single, double, and triple treatments. Bars represent an average (mean)±SE and Asterisks depict statistically significant differences compared to C1498 injected groups (*p<0.05 vs. C1498 injected group, n=12). WT: wild type, C1498: C1498 injected, P: peptide, M: MAZ51, A: ara-C.

Article Snippet: The murine myeloid leukemia cell line C1498 (a murine AML cell line, TIB-49; ATCC, Manassas, VA, USA) was used.

Techniques: Injection

Journal: International Journal of Stem Cells

Article Title: Peptides Targeting Fms-Related Tyrosine Kinase-4 Activate the Function of Natural Killer Cells in Acute Myeloid Leukemia

doi: 10.15283/ijsc21083

Figure Lengend Snippet: The effects of peptide targeted FLT-4 in AML mouse model. The transcriptional level of cytolytic factors including LAMP1, perforin, granzyme B, and IFN-γ displayed in spleen and liver. Bars represent an average (mean)±SE and Asterisks depict statistically significant differences compared to C1498 injected groups. Data shown represent the means of independent experiment with duplicate (*p<0.05 vs. C1498 injected group, n=4). WT: wild type, C1498: C1498 injected, P: peptide, M: MAZ51, A: ara-C.

Article Snippet: The murine myeloid leukemia cell line C1498 (a murine AML cell line, TIB-49; ATCC, Manassas, VA, USA) was used.

Techniques: Injection

Journal: International Journal of Stem Cells

Article Title: Peptides Targeting Fms-Related Tyrosine Kinase-4 Activate the Function of Natural Killer Cells in Acute Myeloid Leukemia

doi: 10.15283/ijsc21083

Figure Lengend Snippet: Combinational therapies with peptide and ara-C effectively suppress leukemia blast cells in AML mice. (A) H&E staining. BM, spleen and liver from C1498 cells injection mice showed successful engraftment of leukemia cells. Insets (magnification, ×40) show enlargement of cells within the main images (magnification, ×10). (B) Statistical analysis for the upper panel of a single treated group. And the lower panel for double-, triple treatment groups. Bars represent an average (mean)±SE, and asterisks represent statistically significant differences compared to C1498 injected group The indicated values are the averages calculated from at least 5 randomly selected fields of each group of 3 independent experiments (*p<0.05; **p<0.01; vs. C1498 injected group, n=12). C1498: C1498 injected, P: peptide, M: MAZ51, A: ara-C.

Article Snippet: The murine myeloid leukemia cell line C1498 (a murine AML cell line, TIB-49; ATCC, Manassas, VA, USA) was used.

Techniques: Staining, Injection

Journal: International Journal of Stem Cells

Article Title: Peptides Targeting Fms-Related Tyrosine Kinase-4 Activate the Function of Natural Killer Cells in Acute Myeloid Leukemia

doi: 10.15283/ijsc21083

Figure Lengend Snippet: Comparison of survival over time in the groups. No significant differences in the survival rate were detected between each group, however, dual and triple treated groups displayed a long life span, compared to that of C1498 groups and single only treated groups. It suggests the benefits of peptide and ara-C in AML treatment. WT: wild type, C1498: C1498 injected, P: peptide, M: MAZ51, A: ara-C.

Article Snippet: The murine myeloid leukemia cell line C1498 (a murine AML cell line, TIB-49; ATCC, Manassas, VA, USA) was used.

Techniques: Comparison, Injection

Journal: Frontiers in Pharmacology

Article Title: Visomitin as a differentiation-inducing therapeutic agent through SYK inhibition in AML

doi: 10.3389/fphar.2026.1741351

Figure Lengend Snippet: Visomitin induces cell differentiation in AML. (A) CD14 expression was detected by FACS analysis after the Visomitin treatment (500 nM) for 72 h in U937 and HL 60 cell lines. (B) Giemsa staining results showing cell differentiation in AML cell lines in response to a 500 nM Visomitin treatment for 72 h in HL60 and U937 cell lines. (C) Phagocytosis was assessed by flow cytometry and fluorescence microscopy in U937 cells, with representative images shown from three independent experiments. (D) Oxidative burst was assessed by FACS analysis in U937 cells following 72-h treatment with 500 nM Visomitin using Dihydrorhodamine-123 staining. (E) Relative expression levels of myeloid differentiation-related genes were measured by qRT-PCR in U937 cells at 72-h intervals post 500 nM Visomitin treatment. (F) Relative expression levels of macrophage gene signatures were measured by qRT-PCR in U937 cells after 72-h treatment with 500 nM Visomitin. The data are presented as mean ± SD (n = 3) for each group and statistical analysis was performed using the Student’s t-test and two-tailed Mann–Whitney U test (*p < 0.05).

Article Snippet: The human AML cell lines HL60, U937 and mouse AML cell line M1 were purchased from Korean Cell Line Bank (Seoul, Korea).

Techniques: Cell Differentiation, Expressing, Staining, Flow Cytometry, Fluorescence, Microscopy, Quantitative RT-PCR, Two Tailed Test, MANN-WHITNEY

Journal: Frontiers in Pharmacology

Article Title: Visomitin as a differentiation-inducing therapeutic agent through SYK inhibition in AML

doi: 10.3389/fphar.2026.1741351

Figure Lengend Snippet: Visomitin did not increase mtROS level in normal myeloid cells. Mitochondrial ROS levels and apoptosis were assessed by flow cytometry following treatment with 500 nM Visomitin for 24 h. Analyses were performed on (A) normal mouse bone marrow cells, (B) myeloid cells (CD45 + /CD11b + double-positive cells isolated from bone marrow), and (C) the mouse AML cell line M1. Apoptotic cells were identified by Annexin V staining. Data are presented as mean ± SD (n = 3 per group). Statistical significance was determined using the two-tailed Mann–Whitney U test (*p < 0.05).

Article Snippet: The human AML cell lines HL60, U937 and mouse AML cell line M1 were purchased from Korean Cell Line Bank (Seoul, Korea).

Techniques: Flow Cytometry, Isolation, Staining, Two Tailed Test, MANN-WHITNEY

Journal: Oncology Letters

Article Title: Circular RNA ATAD1 is upregulated in acute myeloid leukemia and promotes cancer cell proliferation by downregulating miR-34b via promoter methylation

doi: 10.3892/ol.2021.13060

Figure Lengend Snippet: Circ-ATAD1 overexpression increases acute myelogenous leukemia cell proliferation through miR-34b. Role of circ-ATAD1 and miR-34b in regulating (A) Kasumi-3 and (B) Kasumi-6 cell proliferation was analyzed by BrdU assay. Representative images of (C) Kasumi-3 and (D) Kasumi-6 cells are displayed. Scale bar, 20 µm. Blue signals indicate DAPI staining; red signals indicate Brdu signaling Normalized data of three biological replicates are presented. Mean values of three replicates are presented. *P<0.05. circ, circular RNA; miR, microRNA; NC, negative control.

Article Snippet: Kasumi-3 and Kasumi-6 AML cell lines were purchased from the ATCC, and cultured in RPMI-1640 medium (10% FBS) at 37°C in an incubator with 5% CO 2 and 95% humidity.

Techniques: Over Expression, BrdU Staining, Staining, Negative Control